GSM2698456: OE Cntl-NE-1_nuclear_RNA; Homo sapiens; RNA-Seq - SRA

SRX2992320: GSM2698456: OE Cntl-NE-1_nuclear_RNA; Homo sapiens; RNA-Seq
1 ILLUMINA (HiSeq X Ten) run: 34.1M spots, 10.2G bases, 4.2Gb downloads

Submitted by: NCBI (GEO)

Study: hMTR4 plays a central role in creating balanced nuclear RNA pools for degradation and export VI

PRJNA393570 • SRP111407 • All experiments • All runs

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To examine whether the competition between hMTR4 with ALYREF is important for the specific exosome recruitment, we performed stranded RNA-seq using rRNA-depleted nuclear RNAs isolated from ALYREF and control overexpression cells. Overall design: rRNA-depleted RNAs isolated from control, ALYREF overexpression HeLa cells were generated by deep sequencing, using Illumina HiSeq X Ten.

Sample: OE Cntl-NE-1_nuclear_RNA

SAMN07337631 • SRS2344267 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: HiSeq X Ten

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: RNA was isolated from HeLa with TRIzol (Invitrogen). 1ug of nuclear RNA was depleted with rRNA and constructed sequencing libraries. Libraries were generated with TruSeq Stranded Total RNA Sample Prep Kit (Illumina) according to the manufacturer’s instructions.

Experiment attributes:

GEO Accession: GSM2698456

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 34.1M spots, 10.2G bases, 4.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR5813827	34,124,084	10.2G	4.2Gb	2017-12-15

ID:: 4263210

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